Ryan R. Cochrane

Ryan R. Cochrane

Biochemistry Ph.D Candidate University of Western Ontario

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A list of all the posts and pages found on the site. For you robots out there is an XML version available for digesting as well.

Pages

Posts

Future Blog Post

less than 1 minute read

Published:

This post will show up by default. To disable scheduling of future posts, edit config.yml and set future: false.

Blog Post number 4

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 3

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 2

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 1

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

portfolio

publications

Designer Sinorhizobium meliloti strains and multi-functional vectors enable direct inter-kingdom DNA transfer

Published in PLoS One, 2019

This paper establishes S. meliloti as a effective microbial host to GC-rich DNA. This research will enable manipulation of soil microbiomes, and cloning of previously difficult DNA.

Recommended citation: SL. Brumwell, MR. MacLeod, T. Huang, RR. Cochrane, RS. Meaney, M. Zamani, O. Matysiakiewicz, KN. Dan, P. Janakirama, DR. Edgell, TC. Charles, TM. Finan, BJ. Karas. (2019). "Designer Sinorhizobium meliloti strains and multi-functional vectors enable direct inter-kingdom DNA transfer." PLoS ONE. 14(7): e0219562. https://ryanrcochrane.github.io/files/paper1.pdf

Rapid method for generating designer algal mitochondrial genomes

Published in bioRxiv, 2019

This paper demonstrates the methods for cloning and engineering of mitochondrial genomes from Phaeodactylum tricornutum. The mitochondrial genome was cloned in yeast and bacteria and found no significant host burden. Following research will detail methods for mitochondrial DNA delivery to P. tricornutum. Together, these techonlogies will estalish P. tricornutum as a model system for studying the mitochondria of diatoms and allow easy installation of biosynthetic pathways.

Recommended citation: RR. Cochrane, SL. Brumwell, MPM. Soltysiak, S. Hamadache, JG. Davis, J. Wang, SQ. Tholl, P. Janakirama, DR. Edgell, BJ. Karas. (2019). "Rapid method for generating designer algal mitochondrial genomes." bioRxiv. http://ryanrochrane.github.io/files/paper2.pdf

Cloning of Thalassiosira pseudonana’s Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli

Published in Biology, 2020

This paper extends the methods demonstrated for cloning algal mitochondrial genomes to a second species: Thalassiosira pseudonana. Additionally, it assesses the effects of harboring a mitochondrial genome as a plasmid in yeast and bacteria.

Recommended citation: RR. Cochrane, SL. Brumwell, A. Shrestha, DJ. Giguere, S. Hamadache, GG. Gloor, DR. Edgell, and BJ. Karas. (2020). "Cloning of Thalassiosira pseudonana’s Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli." Biology. 9(11): 358. https://ryanrcochrane.github.io/files/paper3.pdf

talks

teaching

Be-Al-U-Can B

Workshop, University of Western Ontario, 2017

This club is a science outreach program for middle school students. Graduate students prepare and present a science tutorial in the student’s classroom in the hopes of sparking student interest and participation in basic science concepts. Another goal of the club is to spread awareness about ongoing local research.

Raising Hope Through Science

Workshop, Victoria Hospital for Sick Children, 2017

Graduate students meet to develop an array of simple science experiments. Then in pairs graduate students go to the local children’s hospital to teach a basic science concept sick children. Also, it provides children who are often isolated an opportunity to engage in activities that provide joy and mental stimulation.

SynBio Hacks Case Competition

Mentor, University of Western Ontario, 2018

Groups composed of graduate and undergraduate students were given a real world problem and asked to find a solution using synthetic biology that was both realistic and feasible. Out of multiple proposals a panel of professors in related fields determined my teams proposal the most likely to succeed and awarded us first place.