Ryan R. Cochrane

Ryan R. Cochrane

Biochemistry Ph.D Candidate University of Western Ontario

Publications

Cloning of Thalassiosira pseudonana’s Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli

Published in Biology, 2020

This paper extends the methods demonstrated for cloning algal mitochondrial genomes to a second species: Thalassiosira pseudonana. Additionally, it assesses the effects of harboring a mitochondrial genome as a plasmid in yeast and bacteria.

Recommended citation: RR. Cochrane, SL. Brumwell, A. Shrestha, DJ. Giguere, S. Hamadache, GG. Gloor, DR. Edgell, and BJ. Karas. (2020). "Cloning of Thalassiosira pseudonana’s Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli." Biology. 9(11): 358. https://ryanrcochrane.github.io/files/paper3.pdf

Rapid method for generating designer algal mitochondrial genomes

Published in bioRxiv, 2019

This paper demonstrates the methods for cloning and engineering of mitochondrial genomes from Phaeodactylum tricornutum. The mitochondrial genome was cloned in yeast and bacteria and found no significant host burden. Following research will detail methods for mitochondrial DNA delivery to P. tricornutum. Together, these techonlogies will estalish P. tricornutum as a model system for studying the mitochondria of diatoms and allow easy installation of biosynthetic pathways.

Recommended citation: RR. Cochrane, SL. Brumwell, MPM. Soltysiak, S. Hamadache, JG. Davis, J. Wang, SQ. Tholl, P. Janakirama, DR. Edgell, BJ. Karas. (2019). "Rapid method for generating designer algal mitochondrial genomes." bioRxiv. http://ryanrochrane.github.io/files/paper2.pdf

Designer Sinorhizobium meliloti strains and multi-functional vectors enable direct inter-kingdom DNA transfer

Published in PLoS One, 2019

This paper establishes S. meliloti as a effective microbial host to GC-rich DNA. This research will enable manipulation of soil microbiomes, and cloning of previously difficult DNA.

Recommended citation: SL. Brumwell, MR. MacLeod, T. Huang, RR. Cochrane, RS. Meaney, M. Zamani, O. Matysiakiewicz, KN. Dan, P. Janakirama, DR. Edgell, TC. Charles, TM. Finan, BJ. Karas. (2019). "Designer Sinorhizobium meliloti strains and multi-functional vectors enable direct inter-kingdom DNA transfer." PLoS ONE. 14(7): e0219562. https://ryanrcochrane.github.io/files/paper1.pdf